Class-Vp Shimadzu Software Solutions To
Class-Vp Shimadzu Serial Dilutions From
Class-Vp Shimadzu Manual Injector Fitted

Class-Vp Shimadzu Software Solutions To

Since then, Shimadzu has been a leader in the field of processing of chromatographic data, offering a range of software solutions to fit any laboratorys requirements.

Shimadzu has also advanced over the years in the field of PC based data systems, progressing from a DOS-based system to todays Labsolutions Series Workstation.

In the pharmaceutical industry, compliance to regulations and guidelines, such as CSV and PICS GMP, U.S.

FDA 21 CFR Part 11, Data Integrity and so on, and the proper, efficient management of instruments and analytical data are required.

With this background, faster, more efficient management of instruments and data is essential.

LabSolutions is a network-compatible analysis data system capable of meeting these needs.

To the best of our knowledge no method is available in literature which can analyze these drugs simultaneously, so there was a need to develop a method which could simultaneously determine all these drugs.

In HPLC, diltiazem and statins are chromatographed using acetonitrile water (85: 15 vv, pH 2.6 0.02) as the mobile phase at a flow rate of 1.0 mL min 1 at ambient temperature.

The separation is carried out on a Hiber, 250-4.6 RP-18 column, equipped with a UVvisible detector at 230 nm.

All the statins eluted at a different retention time and each showed good resolution from diltiazem.

The method has been successfully applied to pharmaceutical formulations because no chromatographic interferences from the tablet excipients are found.

The linearity is found to be in the range 0.62520 g mL 1.

The suitability of the method for the quantitative determination of the drugs is proven by validation in accordance with the requirements laid down by the International Conference on Harmonization (ICH) guidelines.

The validation results, together with statistical treatment of the data, demonstrated the reliability of this method.

These factors are related to family history, sex, age, obesity and diabetes.

Coadministration of antihypertensive, hypolipemiant and antidiabetic drugs is frequently used in treatment.

One of the most used combinations consists of a synergic association of a diuretic ( chlorthalidone, hydrochlorothiazide, etc.

Therefore, it has been hypothesized that coadministration of an antihypertensive agent and statin might be an effective therapeutic option for multiple cardiovascular risk factors and concomitant management of elevations in blood pressure and LDL cholesterol.

Nearly all these assay methods are based on either HPLC or GC techniques 38 like assay scheme for rosuvastatin, 39 simvastatin 40,41 and atorvastatin.

An HPLC method for simultaneous determination of HMG-CoA reductase inhibitors in pharmaceutical formulations and plasma or serum has been reported.

These methods have been applied to pharmaceuticals and plasma samples.

The present study was conducted to analyze the diltiazem and statins in pharmaceutical formulations and human serum.

We present the development and validation of a HPLC assay using UV detector for measurement and allowed determination of each agent without the need for development of separate and distinct methods for each analyte.

The chromatographic system was integrated via Shimadzu model CBM-102 to P-IV computer loaded with Shimadzu CLASS-VP software (Version 5.03) for data acquisition and mathematical calculations.

Class-Vp Shimadzu Manual Injector Fitted

Rheodyne manual injector fitted with a 20 L loop, a Hiber, RT 250-4.6 Purospher STAR RP-18 column and DGU-14 AM on-line degasser and Mettler Toledo electronic balance, microlitre syringe and micropore filtration assembly were used in this study.

Rosuvastatin (X-plended 20 mg), atorvastatin (Atopitar 10 mg) and simvastatin (Atcol 10 mg) Pharm Evo (Pvt.) Ltd., Atco Pharma(Pvt.) Ltd., and Geofman Pharma (Pvt.) Ltd., respectively, were purchased locally.

Reference standards of all these statins were supplied by lab-9 of the Department of Chemistry at the University of Karachi.

Each product was labelled and had an expiry date of not less than 365 days at the time of study.

Stock solutions of diltiazem and statins were prepared in the mobile phase.

All solutions were filtered (0.45 m) and degassed using a sonicator.

Class-Vp Shimadzu Serial Dilutions From

Seven concentrations of each drug were prepared by making serial dilutions from stock solutions.

For the assay preparation, the content of 20 tablets were powdered, a weighed portion of the powder equivalent to a suitable amount of drug (according to the labeled claims) was transferred into a 50 mL volumetric flask.

The drug was fully dissolved in mobile phase and then diluted with this solvent up to the mark, seven dilutions of each drug were prepared, a portion of this solution was filtered through a disposable 0.45 m filter and then injected.

After thawing, serum was deprotonated by acetonitrile and spiked daily with working solutions to produce desired concentrations of diltiazem and statins.

A 20 L volume of each sample was injected and chromatographed under the above conditions.

The mobile phase consisted of acetonitrile water (85: 15 vv) with pH adjusted to 2.6 0.02 with phosphoric acid (85) and pump at a flow rate of 1 mL min 1, sample volume of 20 L was injected in triplicate onto the HPLC column and effluents were screened over the dative UV region at 230 nm.